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Basic Cell Culture
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ISBN
:
9780195676020
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Publisher
:
Oxford University Press India
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Subject
:
Technology: General Issues
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Binding
:
Paperback
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Pages
:
408
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Year
:
2005
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565.0
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440.0
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Description
The aim of this book is to guide the newcomer progressively through all those areas which nowadays are basic to the performance of cell culture. The book should also prove useful to the experienced worker when entering a new field, setting up a new laboratory, or as a source of reference on basic techniques. The use of animal, including human, cellculture has expanded enormously during the last 25 years, with new applications appearing all the time. The aim of this book is to guide the newcomer progressively through all those areas which nowadays are basic to the performance of cell culture. The book should also prove useful to the experienced worker when entering a new field, setting up a new laboratory, or as a source of reference on basic techniques. Revised and updated chapters cover topics such as equipping a cell culture laboratory, sterilization, culture media, basic culture technique, cell cloning, the isolation of new cell lines, contamination procedures, and good laboratory practice. There is a brand new chapter on the microscopy of living cells, and an appendix of online resources for cell biology. Key Features AValuable to both newcomers and experienced researchers. Fully revised and updated. Demonstrates techniques of great importance in biotechnology. Provides a grounding to more advanced texts in the Practical Approach Series. AboutThe Author John Davis Bio-Products Laboratory, Elstree, Hertfordshire, UK Table Of Contents Caroline Wigley: The Cell Culture Laboratory. Peter Roberts: Sterilization. T Cartwright and G P Shah: Culture Media. Ian Dobbie and Daniel Zicha: Microscopy of Living Cells. James McAteer and John M Davis: Basic cell culture and the maintenance of cell lines. Caroline McDonald: Primary culture and the establishment of cell lines. J D Sato et al: Specific cell types and their requirements. John Clarke, Alison Porter, Robin Thorpe, and John M Davis: Cloning. B J Bolton, P Packer and A Doyle: The quality control of cell lines. Barbara Clitherow: Good laboratory practice in the cell culture laboratory. Appendix1 J Dow: Online resources for cell biology.
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