Biotechnology: Principles and Applications

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Biotechnology: Principles and Applications covers broad vistas of biotechnology, for students providing a sound basis of understanding of various aspects of this ever-growing field. This comprehensive book includes a wide coverage of topics needed to appreciate the principles and applied aspects of biotechnology. Key Features Core Techniques including DNA Sequencing, Microarray Technology, Protein Engineering and Mutagenesis Recombinant DNA Technology and Gene Cloning Vectors and Expression Systems for Heterologous Proteins Fermentation Technology and Down-stream Processing Transgenic Animals Technology and Applications Plant Cell Culture, Crop Improvement & Biofertilizers Environmental Biotechnology Including Utilization of Biomass, Bioremediation, Biomining etc. Biotechnology in Medicine and Healthcare Bioethical andLegal Issues in Biotechnology Table of Contents Preface  Section-A: Core Techniques in Biotechnology – Introduction  Enzyme Biotechnology  Isolation and Purification of DNA  Genomics and DNA Sequencing Techniques  Synthesis of Oligonucleotides  Microarray Technology  Purification and Structure Visualization of Proteins  Protein Engineering and Mutagenesis  Proteomics  Section-B: DNA Technology – Enzymesin DNA Technology  Gene Cloning: Genetic Engineering  Nucleic Acid Hybridization  Recombinant DNA Libraries  Polymerase Chain Reaction and its Applications  Section-C: Cloning Vectors and Expression of recombinant Proteins – Plasmids and Transposons  Bacteriophages as Vectors  Other Specialized Vectors  Eukaryotic Vectors  Prokaryotic Expression Systems  Eukaryotic Expression Systems  Section D: Fermentation: Principles and Technology – Fermentation Technology  Bioreactor Design and Instrumentation  Down-stream Processing  Production of Fermented Foods and Beverages  Production of Fuels and Chemicals  Section-E: Animal Biotechnology – Animal Cell Culture and Characterization of Cell Lines  Application of Animal Cell Culture  Immunotherapy and Monoclonal Antibodies  Transgenic Animal Technology  Gene Transfer Technology  Section-F: Plant Biotechnology – Plant Cell & Tissue Culture Technology  Application of Cell and Tissue Culture  Transgenic Plants and Crop Improvement  Agriculture Biotechnology  Section-G: Environmental Biotechnology – Bioremediation  Utilization of Biomass  Biotechnology of Environmental Pollutants  Biomining and Bioleaching  Section-H: Biotechnology in Medicine & Healthcare – Biotechnology in Medicine and Healthcare  Gene Therapy  Xenotransplantation  Legal and Ethical Issues in Biotechnology  Recommended Reading  Index.