Laboratory Manual For Practical Biochemistry

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This book fulfills the need for a complete and comprehensive book on orientation towards various aspects of practical biochemistry. A clear-cut practical concept has been presented in respect of all qualitative and quantitative experiments. Chapters on demonstration experiments such as colorimetry, electrophoresis, chromatography etc. are also included. Questions at the end of each chapter and normal values of various biochemical parameters in blood and urine are incorporated. A concise, exam-oriented, knowledge-based laboratory manual for undergraduate students studying biochemistry. Table Of Contents Qualitative Experiment 1. Carbohydrates 2. Reactions of carbohydrates 3. Scheme for identification of unknown carbohydrates 4. Proteins 5.Color reactions of proteins 6. Precipitation reactions of proteins 7. Scheme for identification of unknown proteins 8. Reactions of non-protein nitrogenous substances 9. Scheme for identification of physiologically important substances 10. Urine analysis (Normal urine) 11. Analysis of abnormal constituents in urine 12. Analysis of gastric juice 13. Analysis of saliva Quantitative Experiment 14. Sample collection and preservation 15. Estimation of blood sugar by Folin-Wu method 16. Estimation of blood glucose by O-toluidine method 17. Estimation of blood urea by diacetyl monoxime (DAM) method 18. Estimation of serum proteins and albumin/globulin ratio by Biuret method 19. Estimation of serum albumin by Dye-binding method 20. Estimation of serum inorganic phosphate by Fiske-Subba Row method 21. Estimation of serum cholesterol by Zak’s method Laboratory Manual for Practical Biochemistry 22. Estimation of serum uric acid by Caraway method 23. Estimation of serum transaminases by Reitaman and Frankel method 24. Estimation of serum bilirubin by Malloy-Evelyn method 25. Estimation of serum creatinine by Jaffe’s alkaline picrate method 26. Estimation of urine creatinine by Jaffe’s alkaline picrate method Demonstration Experiment 27. Colorimetry — general principle and procedure 28. Electrophoresis 29. Chromatography 30. Preparation of hemin crystals Oral Glucose Tolerance Test (ORAL GTT) Case Reports Normal Values Of Biochemical Parameters Index