METHODS IN ENZYMOLOGY: GUIDE TO MOLECULAR CLONING TECHNIQUES

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This self-contained, state-of-the-art manual is designed to meet the needs of the student, experienced researcher, and newcomer to the molecular biology discipline seeking an efficient means of obtaining a clone. Available in an easy-to-use spiral-bound edition, Guide to Molecular Cloning Techniques progresses from basic techniques underlying much of recombinant DNA technology to a series of sections addressing commonly met problems. Key Features* Comprehensive protocols compiled specifically for this volume* Among the topics covered:* Genomic cloning* Preparation and characterization of mRNAcDNA cloning* Screening libraries* Identification and characterization of clones* Methods written by 87 experts* Editors' notes, overviews, and extensive cross-referencing* Process Guide, a compendium of basic processes in molecular biology indexed for at-a-glance accessibility* A problem-solving quantitative approach* Emphasis on strategies for choosing alternative methods