Principles and Techniques of Biochemistry and Molecular Biology

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This best-selling undergraduate textbook provides an introduction to key experimental techniques from across the biosciences. It uniquely integrates the theories and practices that drive the fields of biology and medicine, comprehensively coveringboth the methods students will encounter in lab classes and those that underpin recent advances and discoveries. Its problem-solving approach continues with worked examples that set a challenge and then show students how the challenge is met. New to this edition are case studies, for example, that illustrate the relevance of the principles and techniques to the diagnosis and treatment of individual patients. Coverage is expanded to include a section on stem cells, chapters on immunochemical techniques and spectroscopy techniques, and additional chapters on drug discovery and development, and clinical biochemistry. Experimental design and the statistical analysis of data are emphasized throughout to ensure students are equipped to successfully plan their own experiments and examine the results obtained. Table of Contents 1. General principles K. Wilson 2. Cell culture techniques A. R. Baydoun 3. Centrifugation K. Ohlendieck 4. Microscopy S. W. Paddock 5. Molecular biology, bioformatics and basic techniques R. Rapley 6. Recombinant DNA and genetic analysis R. Rapley 7. Immunological techniques R. Burns 8. Protein structure, purification, characterisation and functional analysis J. M. Walker 9. Mass spectrometric techniques A. Aitken 10. Electrophoretic techniques J. Walker 11. Chromatographic techniques K. Wilson 12. Spectroscopic techniques I A. Hofmann 13. Spectroscopic techniques II A. Hofmann 14. Radioisotope techniques R. Slater 15. Enzymes K. Wilson 16. Principles of clinical biochemistry J. Fyffe and K. Wilson 17. Cell membrane receptors K. Wilson 18. Drug discovery and development K. Wilson.