Laboratory Manual of Food Microbiology

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Principles of Laboratory Food Microbiology serves as a general laboratory guide for individuals in quality control, quality assurance, sanitation, and food production who need to increase their knowledge and skills in basic and applied food microbiology and food safety. This is a very useful book for food industry personnel with little or no background in microbiology or who need a refresher course in basic microbiological principles and laboratory techniques. Focusing on basic skill-building throughout, the book provides a review of basic microbiological techniques - media preparation, aseptic techniques, dilution, plating, etc. - followed by analytical methods and advanced tests for food-borne pathogens. It reviews basic microbiology techniques to evaluate the microbiota of various foods and enumerate indicator microorganisms. It emphasize on conventional cultural techniques. It also focuses on procedures for detecting pathogens in food, offering students the opportunity to practice cultural and biochemical methods. The final section discusses beneficial microorganisms and their role in food fermentations, concentrating on lactic acid bacteria, acetic acid bacteria and yeast. It provides an ideal text companion for an undergraduate or graduate laboratory course, offering professors an authoritative frame of reference for their own supplementary materials and to the food processing industry personnel, Government and private organization linked with food processing and microbial quality of the processed product. The book is an essential text for microbiologists working in the food industry, quality assurance personnel and academic researchers. Contents: 1. Microbiology Laboratory Orientation / 2. Sterilization and Disinfection / 3. Media Preparation / 4. Microscope / 5. Aseptic Culture Technique / 6. Isolating Bacteria: Pure Culture Technique / 7. Enumeration of Bacteria / 8. Colony Description / 9. Microscopic Examination of Living Bacteria / 10. Antibiotic Sensitivity Testing / 11. Biochemical Tests for Identification of Bacteria / 12. Isolation of Moulds / 13. Enumeration of Yeasts and Moulds in Foods / 14. Microscopic Examination of Moulds / 15. Microbiological Examination of various Food Materials / 16. Microbial Examination of Canned Foods / 17. Water Microbiology / 18. Solation and Identification of Common Food Borne Pathogens / 19. Microbial Standards for Different Foods and Drinking Water / 20. Chemical Food Preservatives / 21. Heat Preservation of Microorganisms / 22. Disinfectants and Antiseptics / 23. Preservation of Microbial Cultures / 24. Food Fermentations / 25. Good Manufacturing Practice Codex Alimentarius and Haccp / 26. Media and Reagents / Index